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CONTRAST CURATIVE EFFECT REPORT FOR MEDICINE COW CLINICAL MASTITIS & ITS SAFETY TEST

ZU ZHIDA,HAN ZHANGXING, GE JIANMING: DAIRY COW BREED CENTRAL MODEL FARM SAO GUOXIONG: HANGZHOU DAIRY CO.,MILK FACTORY ZIP CODE: 310022 I.

Masfrigao vs Penicilin G Potassium & Streptomycin Sulface Dated:Apr.11--May 10,99. MATERIALS & METHODS:

A. CASE SOURCE: THE PATHOGENIC COWS FROM HANGZHOU DAIRY HERD WHICH HAVE CLINICAL MASTITIS SIGNS BUT WITHOUT SYSTEMIC REACTION.TOTAL 50 INFECTED QUARTERS OF 50 COWS.

B. MEDICINE: MASFRIGAO MANUFACTURED BY Veterinary Medicine .100ML/BOTTLE

CONTROL GROUP:1.PENICILLIN G POTASSIUM กก40IU

2.STREPTOMYCIN SULFATE กก100IU

METHODS: MASFRIGAO : INFUSION 15ML INTO THE GLAND FROM THE TEAT CANAL, TWICE A DAY.

ANTIBIOTICS: 4M. UNIT OF PENICILLIN G. POTASSIUM & 4M. UNIT OF TREPTOMYCIN SULFATE,INTRAMUSCULAR INJECTION. TWICE A DAY.

THERAPEUTIC EVALUTION: (DIVIDING INTO: A.B.C.)

A. RECOVERY:CLINICAL MASTITIS SIGNS DISAPPEARED,HAVE NO MILK CLOT IN INITIAL 3 MILK. THE OUTPUT OF MILK GOING UP & NORMAL.

B. EFFECTIVE:CLINICAL MASTITIS SIGNS ABATEMENT, OR NO MORE EXACERBATION, BUT THERE ARE MILK CLOTS IN INITIAL MILK.

C. INEFFICACY: CLINICAL MASTITIS SIGNS AS SAME AS BEFORE OR EXACERBATION. CONTRAST ANALYSIS:

  DOSE COWS RECOVERY
*
EFFECTIVE
**
MASFRIGAO 15ML 27 A.27 3.81 (1.9DAYS) B.0 100%
ANTIBIOTICS 4M.+4M.UNIT 23 A.21 3.72(1.86DAYS) B.2 91.3%

* AVERAGE TIME OF infusion (eg. Recovered after 3.81 infusions(average),means,recovered after 1.9days)

** EFFECTIVE CURE RATE.FROM ABOVE DATA WE CAN SEE, DOSE OF MASFRIGAO & ANTIBIOTIC WERE NO DIFFERENTS (3.81~3.72) (P>0.05) BUT CURATIVE RATE IS HIGHER 8.7%. DIFFERENT RATE CLEAR(P<0.01).

II. Masfrigao vs Procaine Benzylpenicilin Oil Dated:Apr.14--May 12,99.

MATERIALS & METHODS:

A. CASE SOURCE: THE PATHOGENIC COWS FROM SHANGHAI FX JK DAIRY HERD.WHICH HAVE CLINICAL MASTITIS SIGNS BUT WITHOUT SYSTEMIC REACTION.TOTAL 50 INFECTED QUARTERS OF 50 COWS.

B. MEDICINE: MASFRIGAO MANUFACTURED BY VETERINARY MEDICINE, 100ML/BOTTLES

CONTROL GROUP: PROCAINE BENZYLPENICILLIN OIL 100ML

C. METHODS: MASFRIGAO : INFUSION 20ML INTO THE GLAND FROM THE TEAT CANAL,ONE INFUSION A DAY.

ANTIBIOTIC: 15ML PROCAINE BENZYLPENICILLIN OIL INTRAMAMMARY INFUSION. ONE INFUSION A DAY.

THERAPEUTIC EVALUTION: (DIVIDING INTO A.B.C. AS ABOVE)

CONTRAST ANALYSIS:

  DOSE COWS RECOVERY * EFFECTIVE * C * ** ***
MASFRIGAO 20ML 22 A.18 3.17 B.3 8 1 10MORE 81.82 95.45%
ANTIBIOTIC 15ML 28 A.18 4.83 B.8 7.62 2 10MORE 64.29 92.86%

* AVERAGE TIME OF INFUSION (eg. Recovered after 3.17 infusion(average)

** RECOVERY CURE RATE *** EFFECTIVE CURE RATE

MASFRIGAO'S CURATIVE RATE ALSO HIGHER THAN ANTIBIOTICS'.

OTHER RECOVERED CASES OF AFTER MASFRIGAO TREATMENT:

III. HANGZHOU DAIRY CO. HERD II. 57 QUARTERS 2.3 INFUSIONS 100%

IV. HANGJIANG DAIRY HERD 200 QUARTERS 3 98%

V. YUHANG PS DAIRY HERD 300 QUARTERS 3 95%

VI. NANHU DAIRY HERD 38 QUARTERS 2.6 100%

SAFETY LACTOBACILLI (YOGURT) TEST

MATERIALS & METHODS

MILK SOURCE: THE QUARTERS WAS TREATED BY MASFRIGAO & THE QUARTER WAS NO INFECTED BY MASTITIS BUT AT SAME UDDER OF SAME COW. GOT 10 SAMPLES PER QUARTER AFTER 5 HOURS OF TREATMENT. HEALTHY COW, GOT 10 SAMPLES.

TOTAL 30 SAMPLES PUT INTO 250 ML BOTTLES AND PUT THE BOTTLES INTO HOT WATER POT HEAT HEAT THEM UPTO TEMPERATURE 90C THEN KEEP 10 MINUTE. LET THEM COLD TO 45C.

BACTERIA & ITS ACTION: SUPPLIED BY SWEEDEN, NAMED L.BULGARIUS,ST. THERMOPHLIUS(1:1 MIXED) 4% OF THEM PUT INTO 10 ML ABOVE FATLESS MILK (45C ). CULTURE IN A WARM OVEN (KEEP 43C ) TILL SOLIDITY. THEN HAVE A INOCULATION 10 ML TILL 65 C AS A FERMENT SEED. PUT FERMENT SEED INTO SAMPLE MILK (AS 5%) CULTURE THEM INTO WARM OVEN (43 C ). WHEN MILK SAMPLE BECOME STATE COAGULATION AFTER 2 HOURS, 2.5 HOURS & TEST ITS ACIDITY AFTER 2.5 HOURS (10 ML EACH, PUT 3 DRIP OF PHENOLPHTHALEIN TEST WATER, THEN PUT 0.1N NaOH).

RESULT OF TEST:

STATE OF COAGULATION 2H
STATE OF COAGUL'N 2.5H
ACIDITY 2.5H
MASFRIGAO:5 HOURS LATE
+
++
58.1 T
THE TEAT WITHOUTINFUSION:5H.LATE
+
++
58.4 T
HEALTH COW:ALSO 5 H
+
++
58.6 T

. FROM ABOVE DATA, ALTHOUGH THE QUARTER WAS INFUSED MASFRIGAO BUT THE MILK AS THE SAME AS THE QUARTER WITHOUT TREATMENT OF MASFRIGAO MEANS,HEALTHY COW'S MILK. THREE SAPLES HAVE NO DIFFERENTS AFTER 2.5 HOURS (P>0.005), EVIDENCING: MASFRIGAO INFUSION HAVE NO INFLUENCE UPON THE MILK COAGULATE TO YOGURT. EVIDENCE THE MASFRIGAO IS A NON-ANTIBIOTIC RESIDUE MEDICINE.

CONCLUSIONS:

1. MASFRIGAO :CURATIVE EFFECT IS BETTER & FASTER THAN THE ANTIBIOTICS.

2. MASFRIGAO :TO HEAL THE INFECTED COWS,NO RESIDUAL ANTIBIOTIC IN MILK WHEN THE COWS IN THE PERIOD OF TREATMENT.& AFTER.

3. MASFRIGAO :SAFETY FOR MILK YIELD.NO NEED MILK WITHHOLD FOR TREATED QUARTER & NO NEED MILK WITHHOLD FOR OTHER HEALTHY QUARTERS OF SAME COW.(Eg: IF 1 QUARTER ARE TREATED BY MASFRIGAO,THE OTHER 3 QUARTERS' MILK ARE SAFETY ).

MASFRIGAO CAN TREAT 90% MORE OF CLINICAL MASTITIS IN LACTATING COWS.(OTHER ABOUT 5% ARE SEVERE SYSTEMIC CLINICAL SIGNS WITH HIGH TEMPERATURE FEVER, ANORECTIC, CLEAR WATER MILK ).

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